Next Generation Sequencing

Next-generation sequencing has become an essential tool in genetic and genomic analysis. It is increasingly important for experimental scientists to gain the bioinformatics skills required to assess and analyse the large volumes of sequencing data produced by next-generation sequencers. This new next-generation sequencing bioinformatics course aims to equip participants with the essential informatics skills and knowledge required to begin analysing next-generation sequencing data and carry out some of the most common types of analysis.

The programme will cover the algorithmic theory and principles of bioinformatics, along with practical computational sessions using sequence analysis techniques and tools applicable to any species or genome size.

The development of these new massively parallel sequencing technologies has sprung from recent advances in the field of nanotechnology, from the availability of optical instruments capable of reliably detecting and differentiating millions of sources of light or fluorescence on the surface of a small glass slide and from the ingenious application of classic molecular biology principles to the sequencing problem.

NGS Applications:-


  1. Genomic and transcriptomic assemblies
  2. Re-sequencing – SNP/InDel, polymorphisms
  3. Metagenomics/Metatranscriptomics (sp. identification within a population)
  4. Amplicon sequencing (accurate haplotype identification)
  5. Exon capture


  1. Genomic and transcriptomic assemblies
  2. Re-sequencing – SNP/InDel, CNV
  3. RNA-Seq : DGE
  4. ChIP-Seq
  5. miRNA analysis
  6. Metagenomics/Metatranscriptomics